Application
DNA polymerase beta antibody detects DNA polymerase beta protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human POLB-transfected 293T cells 10 % SDS-PAGE DNA polymerase beta antibody dilution: 1:5000Application
DNA polymerase beta antibody detects DNA polymerase beta protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DNA polymerase beta antibody dilution: 1:500.Application
Wild-type (WT) and DNA polymerase beta knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
DNA polymerase beta antibody detects DNA polymerase beta protein at cytoplasm and nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. DNA polymerase beta antibody diluted at 1:250.| Product Name | DNA polymerase beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNA polymerase beta (polymerase (DNA directed), beta)In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. Also see POLA (MIM 312040).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNA polymerase beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineRat (94%), Zebrafish (83%), Xenopus laevis (83%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | POLB polymerase (DNA directed) beta |
|---|---|
| Target | DNA polymerase beta |
| Molecular Weight(MW) | 38 kDa (note) |
| Cellular Localization | Nucleus |
Application
DNA polymerase beta antibody detects DNA polymerase beta protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human POLB-transfected 293T cells 10 % SDS-PAGE DNA polymerase beta antibody dilution: 1:5000
Application
DNA polymerase beta antibody detects DNA polymerase beta protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DNA polymerase beta antibody dilution: 1:500.
Application
Wild-type (WT) and DNA polymerase beta knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
DNA polymerase beta antibody detects DNA polymerase beta protein at cytoplasm and nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. DNA polymerase beta antibody diluted at 1:250.| Positive Control | human POLB-transfected 293T cells (no boiling) , *MCF7 , *MDA-MB-231 , *MDA-MB-361 , *MDA-MB-453 , *T47D , *ZR-75-1 , *293T , *HeLa , *HT29 , *U2OS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
