Application
Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using DNA polymerase gamma antibody(10 μg/ml).Application
Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma antibody at 1:200 dilution.Application
DNA polymerase gamma antibody detects POLG protein by western blot analysis. A. 50 µg Rat testis lysate/extract 5% SDS-PAGE DNA polymerase gamma antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of DNA polymerase gamma protein from MCF-7 whole cell extracts using 5 μg of DNA polymerase gamma antibody . Western blot analysis was performed using DNA polymerase gamma antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 µg of whole cell lysate) A: MCF-7 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | DNA polymerase gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNA polymerase gamma (polymerase (DNA directed), gamma)Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase gamma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanRat |
| Predicted Reactivity | MouseMouse (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ27114 antibody MDP1 antibody MIRAS antibody MTDPS4A antibody MTDPS4B antibody PEO antibody POLG1 antibody POLGA antibody SANDO antibody SCAE antibody POLG antibody "PolG catalytic subunit antibody" DNA polymerase subunit gamma-1 antibody m |
|---|---|
| Molecular Weight(MW) | 140 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using DNA polymerase gamma antibody(10 μg/ml).
Application
Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma antibody at 1:200 dilution.
Application
DNA polymerase gamma antibody detects POLG protein by western blot analysis. A. 50 µg Rat testis lysate/extract 5% SDS-PAGE DNA polymerase gamma antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of DNA polymerase gamma protein from MCF-7 whole cell extracts using 5 μg of DNA polymerase gamma antibody . Western blot analysis was performed using DNA polymerase gamma antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 µg of whole cell lysate) A: MCF-7 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | MCF-7 , rat testis |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
