Application
Sample (30 ug of whole cell lysate) A: PC-3 7.5% SDS PAGE diluted at 1:500| Product Name | DNA polymerase iota antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNA polymerase iota (polymerase (DNA directed) iota)Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNA polymerase iota. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chimpanzee, BovineMouse (85%), Rat (85%), Chimpanzee (98%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RAD30B antibody RAD3OB antibody POLI antibody "polymerase (DNA-directed) iota antibody" RAD30 homolog B antibody eta2 antibody DNA polymerase iota antibody polymerase (DNA directed) iota antibody |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: PC-3 7.5% SDS PAGE diluted at 1:500| Positive Control | PC-3 , *HEK293 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
