Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DNAJB12 antibody at 1:500 dilution.| Product Name | DNAJB12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNAJB12 (DnaJ (Hsp40) homolog, subfamily B, member 12)DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNAJB12. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, BovineMouse (96%), Rat (96%), Xenopus laevis (80%), Chicken (85%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DJ10 antibody DKFZp586B2023 antibody DNAJB12 antibody dnaJ homolog subfamily B member 12 antibody "DnaJ (Hsp40) homolog subfamily B member 12 antibody" |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Membrane |
Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DNAJB12 antibody at 1:500 dilution.| Positive Control | A549 , H1299 , HCT116 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
