Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DNAJC7 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | DNAJC7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNAJC7 (DnaJ (Hsp40) homolog, subfamily C, member 7)DNAJC7 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNAJC7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, BovineMouse (98%), Rat (97%), Chicken (92%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DJ11 antibody DJC7 antibody TPR2 antibody TTC2 antibody DNAJC7 antibody tetratricopeptide repeat protein 2 antibody tetratricopeptide repeat domain 2 antibody TPR repeat protein 2 antibody dnaJ homolog subfamily C member 7 antibody "DnaJ (Hsp40) h |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DNAJC7 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
