Application
Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with hydroxyurea(lane 2) using DNA PKcs(Phospho-Thr2609) antibody| Product Name | DNA-PKcs (phospho Thr2609) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNA-PKcs (phospho Thr2609)The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties |
| Immunogen | Peptide sequence around phosphorylation site of threonine 2609 (V-E-T(p)-Q-A) derived from Human DNA-PK. |
| Modification | p-Thr2609 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DNA-PKcs DNAPK DNPK1 HYRC HYRC1 XRCC7 p350 PRKDC p460 hyper-radiosensitivity of murine scid mutation complementing 1 DNA-PK catalytic subunit DNA-dependent protein kinase catalytic subunit protein kinase DNA-activated catalytic polypeptide |
|---|---|
| Target | DNA-PKcs (phospho Thr2609) |
| Molecular Weight(MW) | 450 (note) |
Application
Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with hydroxyurea(lane 2) using DNA PKcs(Phospho-Thr2609) antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
