Application
Western blot using GeneTex Affinity Purified anti-DNAPKcs antibody shows detection of a 460 kDa band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated Lane 2: Fus1 IR (20Gy, 4h) Lane 3: Fus1 DNAPK inhibitor + IR Lane 4: MO59J (DNAPK-) untreated Lane 5: MO59J IR Lane 6: Fus1 untreated Lane 7: Fus1 IR (20Gy, 4h) Lane 8: Fus1 DNAPK inhibitor + IR Lane 9: MO59J untreated Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 µg of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.| Product Name | DNA-PKcs (phospho Thr2609) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNA-PKcs (phospho Thr2609)DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer. |
| Immunogen | Synthetic peptide - amino acids 2604-2614 of human DNA PKcs - phosphorylated at the pT2609 residue |
| Modification | p-Thr2609 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
|
Not tested in other applications. : |
|
| Species Reactivity | HumanChimpanzee |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HYRC PRKDC DNPK1 DNA PKCS HYRC1 XRCC7 P350 DNAPK |
|---|---|
| Target | DNA-PKcs (phospho Thr2609) |
| Cellular Localization | Nuclear |
Application
Western blot using GeneTex Affinity Purified anti-DNAPKcs antibody shows detection of a 460 kDa band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated Lane 2: Fus1 IR (20Gy, 4h) Lane 3: Fus1 DNAPK inhibitor + IR Lane 4: MO59J (DNAPK-) untreated Lane 5: MO59J IR Lane 6: Fus1 untreated Lane 7: Fus1 IR (20Gy, 4h) Lane 8: Fus1 DNAPK inhibitor + IR Lane 9: MO59J untreated Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 µg of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.| Application Notes |
Not tested in other applications. : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
