Application
DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: DNA-PKcs antibody [C3], C-term dilution: 1:250.Application
DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA-PKcs protein stained by DNA-PKcs antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
DNA PKcs antibody detects DNA PKcs protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA PKcs antibody at a dilution of 1:500.Application
Sample(30 ug whole cell lysate) A:293T B:MOLT4 5% SDS PAGE diluted at 1:1000| Product Name | DNA-PKcs antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DNA-PKcs (protein kinase, DNA-activated, catalytic polypeptide)The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DNA-PKcs. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Dog, ChickenMouse (83%), Dog (83%), Chicken (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DNA-PKcs antibody DNAPK antibody DNPK1 antibody HYRC antibody HYRC1 antibody XRCC7 antibody p350 antibody PRKDC antibody p460 antibody "hyper-radiosensitivity of murine scid mutation complementing 1 antibody" DNA-PK catalytic subunit antibody D |
|---|---|
| Target | DNA-PKcs |
| Molecular Weight(MW) | 469 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: DNA-PKcs antibody [C3], C-term dilution: 1:250.
Application
DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA-PKcs protein stained by DNA-PKcs antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
DNA PKcs antibody detects DNA PKcs protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA PKcs antibody at a dilution of 1:500.
Application
Sample(30 ug whole cell lysate) A:293T B:MOLT4 5% SDS PAGE diluted at 1:1000| Positive Control | 293T , Molt-4 , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
