Application
DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. DNase I antibody [N1C3] diluted at 1:500.Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DNase I antibody [N1C3] detects DNase I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNase I protein stained by DNase I antibody [N1C3] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. DNase I antibody [N1C3] diluted at 1:500.| Product Name | DNase I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNase I (deoxyribonuclease I)This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein corresponding to human DNase I. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | DogDog (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686H0155 antibody DNL1 antibody DRNI antibody FLJ38093 antibody FLJ44902 antibody DNASE1 antibody Dornase alfa antibody deoxyribonuclease-1 antibody "DNase I lysosomal antibody" human urine deoxyribonuclease I antibody deoxyribonuclease I a |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) |
| Cellular Localization | Secreted , Nucleus envelope |
Application
DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. DNase I antibody [N1C3] diluted at 1:500.
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DNase I antibody [N1C3] detects DNase I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNase I protein stained by DNase I antibody [N1C3] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. DNase I antibody [N1C3] diluted at 1:500.| Positive Control | Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
