Application
Immunocytochemistry/Immunofluorescence: Dnmt1 Antibody [ - The Dnmt1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectivelyApplication
Western Blot: Dnmt1 Antibody [ - Analysis using to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 ugs of total protein lysate from untreated T24 degrees Cells. Lane 2: 50 ugs of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.Application
Immunohistochemistry-Paraffin: Dnmt1 Antibody [ - IHC staining of Dnmt1 in mouse spleen using DAB with hematoxylin counterstain.Application
Immunohistochemistry-Paraffin: Dnmt1 Antibody [ - IHC staining of Dnmt1 in mouse small intestine using DAB with hematoxylin counterstain.| Product Name | DNMT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Anti - DNMT1 polyclonalInactivation of tumor suppressor genes is a key component of the development of all common forms of human cancers. Aberrant methylation may contribute to many of the changes, both physiological and pathological, associated with aging, including tumor development. Hypermethylation of CpG island-containing promoters is associated with the inactivation of genes important to tumor angiogenesis and progression. DNA methytransferase (DNMT1) is necessary for the maintenance of the methylation process. |
| Immunogen | This antibody was raised against the first 10 kDa of the N-terminus of the DNMT1 protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P26358 DNMT DNMT1 CXXC9 126375 MCMT 1786 MGC104992 AIM FLJ16293 |
|---|---|
| Target | DNMT1 |
| Tissue Specificity | This antibody is specific for DNMT1. |
Application
Immunocytochemistry/Immunofluorescence: Dnmt1 Antibody [ - The Dnmt1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively
Application
Western Blot: Dnmt1 Antibody [ - Analysis using to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 ugs of total protein lysate from untreated T24 degrees Cells. Lane 2: 50 ugs of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.
Application
Immunohistochemistry-Paraffin: Dnmt1 Antibody [ - IHC staining of Dnmt1 in mouse spleen using DAB with hematoxylin counterstain.
Application
Immunohistochemistry-Paraffin: Dnmt1 Antibody [ - IHC staining of Dnmt1 in mouse small intestine using DAB with hematoxylin counterstain.| Positive Control | T24 cell lysates |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
