Application
Immunohistochemistry: Dnmt3a Antibody [ - Dnmt3a antibody was tested in human testis using DAB with hematoxylin counterstain.Application
Detection of overexpressed DNMT3A level in HCT-116 cells. Lane 1: 40 ug of nuclear extract from untreated HCT-116 cells. Lane 2: 40 ug of Nuclear extract from HCT-116 cells overexpressing DNMT3A.Application
Immunocytochemistry/Immunofluorescence: Dnmt3a Antibody [ - Dnmt3a antibody was tested in Ntera-2 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).| Product Name | DNMT3A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DNMT3AInactivation of tumor suppressor genes is a key component of the development of all common forms of human cancers. Aberrant methylation may contribute to many of the changes, both physiological and pathological, associated with aging, including tumor development. Hypermethylation of CpG island-containing promoters is associated with the inactivation of genes important to tumor angiogenesis and progression. DNA methytransferase (DNMT3a) is necessary for the maintenance of the methylation process. |
| Immunogen | This antibody was raised against amino acids 10-118 of the DNMT3a protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 1788 DNMT3A2 M.HsaIIIA Q9Y6K1 DNMT3a 602769 |
|---|---|
| Target | DNMT3a |
| Tissue Specificity | This antibody is specific for DNMT3a. |
Application
Immunohistochemistry: Dnmt3a Antibody [ - Dnmt3a antibody was tested in human testis using DAB with hematoxylin counterstain.
Application
Detection of overexpressed DNMT3A level in HCT-116 cells. Lane 1: 40 ug of nuclear extract from untreated HCT-116 cells. Lane 2: 40 ug of Nuclear extract from HCT-116 cells overexpressing DNMT3A.
Application
Immunocytochemistry/Immunofluorescence: Dnmt3a Antibody [ - Dnmt3a antibody was tested in Ntera-2 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).| Positive Control | HCT116 cell lysates |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
