Application
Western blot analysis of extracts of various cell lines, using DNMT3A antibody.Application
Immunofluorescence analysis of U2OS cell using DNMT3A antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded mouse spleen using DNMT3A antibody at dilution of 1:200 (x400 lens)Application
Immunohistochemistry of paraffin-embedded mouse brain using DNMT3A antibody at dilution of 1:200 (x400 lens)Application
Immunohistochemistry of paraffin-embedded human stomach using DNMT3A antibody at dilution of 1:200 (x400 lens)Application
Immunohistochemistry of paraffin-embedded human stomach cancer using DNMT3A antibody at dilution of 1:200 (x400 lens)| Product Name | DNMT3A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNMT3ACpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human DNMT3A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:20 - 1:100, IP: 1:20 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DNA (cytosine-5-)-methyltransferase 3 alpha DNMT3A2 M.HsaIIIA |
|---|---|
| Target | DNMT3A |
Application
Western blot analysis of extracts of various cell lines, using DNMT3A antibody.
Application
Immunofluorescence analysis of U2OS cell using DNMT3A antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded mouse spleen using DNMT3A antibody at dilution of 1:200 (x400 lens)
Application
Immunohistochemistry of paraffin-embedded mouse brain using DNMT3A antibody at dilution of 1:200 (x400 lens)
Application
Immunohistochemistry of paraffin-embedded human stomach using DNMT3A antibody at dilution of 1:200 (x400 lens)
Application
Immunohistochemistry of paraffin-embedded human stomach cancer using DNMT3A antibody at dilution of 1:200 (x400 lens)| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:20 - 1:100, IP: 1:20 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
