Application
Immunoprecipitation of DNMT3A protein from 293T whole cell extracts using 5 μg of DNMT3A antibody or DNMT3A antibody . Western blot analysis was performed using DNMT3A antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
DNMT3A antibody immunoprecipitates DNMT3A protein in IP experiments. IP samples: 293T nuclear extract A. 50 μg 293T nuclear extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of DNMT3A protein by 4 μg DNMT3A antibody 5 % SDS-PAGE The immunoprecipitated DNMT3A protein was detected by DNMT3A antibody diluted at 1:2000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
DNMT3A antibody detects DNMT3A protein at nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. DNMT3A antibody dilution: 1:500.Application
DNMT3A antibody detects DNMT3A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and blotted with DNMT3A antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DNMT3A antibody detects DNMT3A protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3A protein stained by DNMT3A antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | DNMT3A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNMT3A (DNA (cytosine-5-)-methyltransferase 3 alpha)CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (85%), Bovine (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DNA (cytosine-5-)-methyltransferase 3 alpha DNMT3A2 M.HsaIIIA |
|---|---|
| Target | DNMT3A |
| Molecular Weight(MW) | 102 kDa (note) (4) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunoprecipitation of DNMT3A protein from 293T whole cell extracts using 5 μg of DNMT3A antibody or DNMT3A antibody . Western blot analysis was performed using DNMT3A antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
DNMT3A antibody immunoprecipitates DNMT3A protein in IP experiments. IP samples: 293T nuclear extract A. 50 μg 293T nuclear extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of DNMT3A protein by 4 μg DNMT3A antibody 5 % SDS-PAGE The immunoprecipitated DNMT3A protein was detected by DNMT3A antibody diluted at 1:2000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
DNMT3A antibody detects DNMT3A protein at nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. DNMT3A antibody dilution: 1:500.
Application
DNMT3A antibody detects DNMT3A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and blotted with DNMT3A antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DNMT3A antibody detects DNMT3A protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3A protein stained by DNMT3A antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , 293T mock , DNMT3A , *CL1-0 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
