Application
Western blot of 20 ug of human caudate lysate showing specific immunolabeling of the ~88k DAT protein using Dopamine Transporter antibody, Extracellular Loop 2 .| Product Name | Dopamine Transporter antibody, Extracellular Loop 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Dopamine Transporter, Extracellular Loop 2The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the extracellular loop 2 region conjugated to KLH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| For IHC: 1:1,000: |
|
| Species Reactivity | HumanPrimate |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Sodium dependent dopamine transporter antibod DAT DAT1 DAT 1 SLC6A3 6531 DA transporter 13162 Solute carrier family6 member3 Solute carrier family 6 member 3 |
|---|---|
| Molecular Weight(MW) | 88 (note) |
Application
Western blot of 20 ug of human caudate lysate showing specific immunolabeling of the ~88k DAT protein using Dopamine Transporter antibody, Extracellular Loop 2 .| Application Notes | For IHC: 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
