Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with DSN1 antibody [N3C3] diluted at 1:500.Application
HeLa cells were fixed with 2% PFA for IF staining. Primary Ab diluted at 1: 1000 with 10% normal donkey serum in PHEM buffer. ACA is used as a marker for inner kinetochore. (Image kindly provided by Drs. J. Herman and J. DeLuca, Colorado State University)| Product Name | DSN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DSN1 (DSN1, MIND kinetochore complex component, homolog (S. cerevisiae))This gene encodes a kinetochore protein that functions as part of the minichromosome instability-12 centromere complex. The encoded protein is required for proper kinetochore assembly and progression through the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DSN1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C20orf172 antibody FLJ13346 antibody KNL3 antibody MGC32987 antibody MIS13 antibody dJ469A13.2 antibody hKNL-3 antibody DSN1 antibody kinetochore-associated protein DSN1 homolog antibody kinetochore null 3 homolog antibody "DSN1 MIND kinetochore |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (6) |
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with DSN1 antibody [N3C3] diluted at 1:500.
Application
HeLa cells were fixed with 2% PFA for IF staining. Primary Ab diluted at 1: 1000 with 10% normal donkey serum in PHEM buffer. ACA is used as a marker for inner kinetochore. (Image kindly provided by Drs. J. Herman and J. DeLuca, Colorado State University)| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
