Application
DUSP8 antibody detects DUSP8 protein by western blot analysis. A. 30 µg Jurkat whole cell extract B. 30 µg Raji whole cell extract 7.5% SDS-PAGE DUSP8 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DUSP8 antibody detects DUSP8 protein at cytoplasm by immunofluorescent analysis. Sample: RD cells were fixed in ice-cold MeOH for 5 min. Green: DUSP8 protein stained by DUSP8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | DUSP8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DUSP8 (dual specificity phosphatase 8)The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DUSP8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C11orf81 HB5 HVH-5 HVH8 dual specificity phosphatase 8 |
|---|
Application
DUSP8 antibody detects DUSP8 protein by western blot analysis. A. 30 µg Jurkat whole cell extract B. 30 µg Raji whole cell extract 7.5% SDS-PAGE DUSP8 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DUSP8 antibody detects DUSP8 protein at cytoplasm by immunofluorescent analysis. Sample: RD cells were fixed in ice-cold MeOH for 5 min. Green: DUSP8 protein stained by DUSP8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
