Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using DUT antibody at 1:500 dilution.Application
Immunoprecipitation of DUT protein from A431 whole cell extracts using 5 μg of DUT antibody . Western blot analysis was performed using DUT antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE diluted at 1:1000| Product Name | DUT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DUT (deoxyuridine triphosphatase)This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DUT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20622 antibody dUTPase antibody DUT antibody dUTP nucleotidohydrolase antibody "deoxyuridine 5'-triphosphate nucleotidohydrolase mitochondrial antibody" dUTP pyrophosphatase antibody deoxyuridine triphosphatase antibody |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using DUT antibody at 1:500 dilution.
Application
Immunoprecipitation of DUT protein from A431 whole cell extracts using 5 μg of DUT antibody . Western blot analysis was performed using DUT antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
