Application
Immunofluorescence analysis of methanol-fixed A431, using DYNC1LI1 antibody at 1:500 dilution.Application
Immunoprecipitation of DYNC1LI1 protein from 293T whole cell extracts using 5 μg of DYNC1LI1 antibody . Western blot analysis was performed using DYNC1LI1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
DYNC1LI1 antibody detects DYNC1LI1 protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. DYNC1LI1 antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:5000| Product Name | DYNC1LI1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DYNC1LI1 (dynein, cytoplasmic 1, light intermediate chain 1)Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human DYNC1LI1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DNCLI1 antibody FLJ10219 antibody LIC1 antibody DYNC1LI1 antibody dynein light chain A antibody cytoplasmic dynein 1 light intermediate chain 1 antibody DLC-A antibody "dynein cytoplasmic light intermediate polypeptide 1 antibody" dynein light ch |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (3) |
Application
Immunofluorescence analysis of methanol-fixed A431, using DYNC1LI1 antibody at 1:500 dilution.
Application
Immunoprecipitation of DYNC1LI1 protein from 293T whole cell extracts using 5 μg of DYNC1LI1 antibody . Western blot analysis was performed using DYNC1LI1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
DYNC1LI1 antibody detects DYNC1LI1 protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. DYNC1LI1 antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:5000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
