Application
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Human Skeletal Muscle stained with anti-Dysferlin antibodyApplication
staining Dysferlin in human skeletal muscle by Immunohistochemistry (FFPE-sections).| Product Name | Dysferlin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DysferlinDysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation. This antibody is used for the characterization of LGMD2B and MM. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| IHC-P: Use at a dilution of 1/50, for 10 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10 minutes followed by cooling at room emperature for 20 minutes. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 231 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LGMD2B FER1L1 253601 DYSF O75923 FLJ00175 Dysferlin 8291 |
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Application
Application
Application
WB Image for Root Catalog#
Application
Human Skeletal Muscle stained with anti-Dysferlin antibody
Application
staining Dysferlin in human skeletal muscle by Immunohistochemistry (FFPE-sections).| Application Notes | IHC-P: Use at a dilution of 1/50, for 10 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10 minutes followed by cooling at room emperature for 20 minutes. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 231 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
