Application
Western blot analysis of Dystrophin expression in 3T3 cell lysate (Lane 1) and rat kidney tissue lysate (Lane 2).| Product Name | Dystrophin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DystrophinDystrophin is one of the actin-binding proteins that are involved in anchoring the cytoskeleton to the plasma membrane. Dystrophin expression is found in muscle brain tissues, where it is located to the inner surface of the plasma membrane. It is suggested that alternative splicing of the caboxy terminus allows dystrophin to interact with a variety of proteins. Loss of dystrophin-associated proteins in Duchenne afflicted muscle is due to the absence of dystrophin rather than to muscle degradation and lack of dystrophin results in the loss of linkage between the cytoskeleton and extracellular matrix. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| The antibody can be used for Western blotting (0.5-4 µg/ml) and in Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~60 and 130 kDa of Dystrophin in samples from human, mouse and rat origins. Reactivity to other species has not been determined.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Dystrophin DXS272 DXS269 DXS268 DXS270 DXS239 DXS142 DXS206 DXS230 CMD3B DMD DXS164 BMD |
|---|
Application
Western blot analysis of Dystrophin expression in 3T3 cell lysate (Lane 1) and rat kidney tissue lysate (Lane 2).| Application Notes | The antibody can be used for Western blotting (0.5-4 µg/ml) and in Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~60 and 130 kDa of Dystrophin in samples from human, mouse and rat origins. Reactivity to other species has not been determined.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol and 0.01% thimerosal |
