Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE E2F6 antibody diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using E2F6 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using E2F6 antibody at 1:500 dilution.| Product Name | E2F6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to E2F6 (transcription factor E2F6)This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human E2F6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, Chimpanzee, BovineMouse (80%), Rat (80%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | E2F-6 antibody MGC111545 antibody E2F6 antibody transcription factor E2F6 antibody "E2F transcription factor 6 isoform 1 antibody" E2F transcription factor 6 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE E2F6 antibody diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using E2F6 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using E2F6 antibody at 1:500 dilution.| Positive Control | A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
