Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
ECH1 antibody detects ECH1 protein at mitochondrion on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. ECH1 antibody dilution: 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ECH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE ECH1 antibody diluted at 1:1000| Product Name | ECH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ECH1 (enoyl CoA hydratase 1, peroxisomal)This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ECH1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, PigRat (82%), Pig (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HPXEL antibody ECH1 antibody "delta(3 5)-Delta(2 4)-dienoyl-CoA isomerase mitochondrial antibody" "enoyl Coenzyme A hydratase 1 peroxisomal antibody" peroxisomal enoyl-CoA hydratase 1 antibody "delta3 5-delta2 4-dienoyl-CoA isomerase antibody" dien |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (2) |
| Cellular Localization | Mitochondrion , Peroxisome |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
ECH1 antibody detects ECH1 protein at mitochondrion on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. ECH1 antibody dilution: 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ECH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE ECH1 antibody diluted at 1:1000| Positive Control | A549 , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
