Application
Immunofluorescence analysis of methanol-fixed HeLa, using ECHS1 antibody at 1:200 dilution.Application
ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody dilution: 1:500.Application
Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody . Western blot analysis was performed using ECHS1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
ECHS1 antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody dilution: 1:500.Application
ECHS1 antibody detects ECHS1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE ECHS1 antibody dilution: 1:1000Application
ECHS1 antibody detects ECHS1 protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 ug PC-3 whole cell lysate/extract C. 30 ug U87-MG whole cell lysate/extract D. 30 ug SK-N-SH whole cell lysate/extract 12 % SDS-PAGE ECHS1 antibody dilution: 1:3000| Product Name | ECHS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ECHS1 (enoyl CoA hydratase, short chain, 1, mitochondrial)The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ECHS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, BovineRat (91%), Pig (86%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SCEH antibody ECHS1 antibody enoyl-CoA hydratase 1 antibody enoyl-CoA hydratase mitochondrial antibody short-chain enoyl-CoA hydratase antibody enoyl Coenzyme A hydratase short chain 1 mitochondrial antibody enoyl CoA hydratase short chain 1 m |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ECHS1 antibody at 1:200 dilution.
Application
ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody dilution: 1:500.
Application
Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody . Western blot analysis was performed using ECHS1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
ECHS1 antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody dilution: 1:500.
Application
ECHS1 antibody detects ECHS1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE ECHS1 antibody dilution: 1:1000
Application
ECHS1 antibody detects ECHS1 protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 ug PC-3 whole cell lysate/extract C. 30 ug U87-MG whole cell lysate/extract D. 30 ug SK-N-SH whole cell lysate/extract 12 % SDS-PAGE ECHS1 antibody dilution: 1:3000| Positive Control | NT2D1 , PC-3 , U87-MG , SK-N-SH , Neuro2A , GL261 , C8D30 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
