Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody [N1C2] dilution: 1:500.Application
ECHS1 antibody detects ECHS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody diluted by 1:5000.Application
Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody [N1C2] . Western blot analysis was performed using ECHS1 antibody [N1C2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. ECHS1 antibody [N1C2] dilution: 1:500.Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody [N1C2] dilution: 1:500.Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody [N1C2] dilution: 1:500.Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: ECHS1 protein stained by ECHS1 antibody [N1C2] diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | ECHS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ECHS1 (enoyl Coenzyme A hydratase, short chain, 1, mitochondrial)The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ECHS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SCEH antibody ECHS1 antibody enoyl-CoA hydratase 1 antibody "enoyl-CoA hydratase mitochondrial antibody" short-chain enoyl-CoA hydratase antibody "enoyl Coenzyme A hydratase short chain 1 mitochondrial antibody" "enoyl CoA hydratase short chain |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (2) |
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody [N1C2] dilution: 1:500.
Application
ECHS1 antibody detects ECHS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody diluted by 1:5000.
Application
Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody [N1C2] . Western blot analysis was performed using ECHS1 antibody [N1C2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. ECHS1 antibody [N1C2] dilution: 1:500.
Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody [N1C2] dilution: 1:500.
Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody [N1C2] dilution: 1:500.
Application
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: ECHS1 protein stained by ECHS1 antibody [N1C2] diluted at 1:200. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
