Application
IHC-P analysis of human rectum tissue using eEF1A1 antibody at a dilution of 1:200.Application
ICC/IF analysis of HeLa cells using eEF1A1 antibody (green) and DAPI (blue).Application
WB analysis of extracts from various cell lines using eEF1A1 antibody.Application
IHC-P analysis of rat testis tissue using eEF1A1 antibody at a dilution of 1:200.Application
IHC-P analysis of human stomach cancer using eEF1A1 antibody at a dilution of 1:200.| Product Name | eEF1A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eEF1A1This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human EEF1A1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:20 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EF-Tu CCS-3 GRAF-1EF eEF1A-1 PTI1 HNGC:16303 LENG7 EEF1A1 EEF-1 EE1A1 EEF1A eukaryotic translation elongation factor 1 alpha 1 Antibod |
|---|---|
| Target | eEF1A1 |
| Molecular Weight(MW) | 50 kDa (note) |
| Tissue Specificity | Based on a BLAST result, it may also cross with human eEF1A2. |
Application
IHC-P analysis of human rectum tissue using eEF1A1 antibody at a dilution of 1:200.
Application
ICC/IF analysis of HeLa cells using eEF1A1 antibody (green) and DAPI (blue).
Application
WB analysis of extracts from various cell lines using eEF1A1 antibody.
Application
IHC-P analysis of rat testis tissue using eEF1A1 antibody at a dilution of 1:200.
Application
IHC-P analysis of human stomach cancer using eEF1A1 antibody at a dilution of 1:200.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:20 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
