| Product Name | EGF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EGFEpidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal and epithelial cells. |
| Immunogen | Highly pure (>98%) recombinant hEGF (human Epidermal Growth Factor) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization:To yield one-half maximal inhibition [ND 50 ] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 μg/ml of this antibody is required. ELISA:To detect hEGF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF. Western Blot:To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | P01133 1950 URG 131530 EGF |
|---|
| Application Notes | Neutralization:To yield one-half maximal inhibition [ND 50 ] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 μg/ml of this antibody is required. ELISA:To detect hEGF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF. Western Blot:To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.4, no preservative, sterile filtered |
