| Product Name | EGFR (phospho Tyr1016) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EGFR ( phospho Y1016 )The EGFR, epidermal growth factor receptor, belongs to a family of receptor tyrosine kinases in mammals which is composed of four members: EGFR (ErB1), ErB2, ErB3, and ErB4. EGFR is an 1210 amino acid residue trans-membrane glycoprotein. It consists of an extracellular ligand binding domain, a single alpha helical transmembrane pass, an intracellular tyrosine kinase domain, and a COOH-terminal region that contains autophosphorylation sites. The binding of specific polypeptide ligands, including EGF, transforming growth factor alpha, betacellulin, heparin-binding EGF, epiregulin, or amphiregulin, results in phosphorylation of multiple tyrosine residues in the COOH-terminal tail, such as tyrosine 869Y, 1016Y, 1092Y, 1110Y, 1125Y, 1172Y, 1197Y, etc. The binding of phosphorylated EGFR tyrosines with downstream signaling proteins initiates multiple signaling cascades that culminate in cell proliferation, migration, protein secretion, differentiation and/or oncogenesis. |
| Immunogen | Synthetic peptide corresponding to EGFR phosphorylated at Tyrosine 1016 (Sequence identical in Human and Rat). |
| Modification | p-Tyr1016 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| ELISA: Use at a dilution of 1/1000. IP: Use 3-5μg for 10^6 cells. WB: Use at a dilution of 1/500. Detects a band of approximately 170 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERBB1 131550 1956 EGFR ERBB mENA P00533 EGFR (phospho Y1016) EGFR (phospho Tyr1016) EGFR (pY1016) EGFR (pTyr1016) EGFR phospho Y1016 EGFR phospho Tyr1016 Phospho EGFR (Y1016) Phospho EGFR (Tyr1016) Phospho EGFR (pY1016) Phospho EGFR (pTyr10 |
|---|---|
| Target | EGFR |
| Tissue Specificity | This antibody is specific for EGFR phosphorylated on Tyrosine 1016. EGFR phosphorylated at sites other than Tyrosine 1016 does not cross react. |
| Cellular Localization | Cell Membrane and Secreted |
| Positive Control | A431 whole cell lysate |
|---|---|
| Application Notes | ELISA: Use at a dilution of 1/1000. IP: Use 3-5μg for 10^6 cells. WB: Use at a dilution of 1/500. Detects a band of approximately 170 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4ºC for short-term storage. Aliquot and store at -20°C for long-term. Avoid repeated freezing and thawing. |
| Storage Buffer | Preservative: 0.02% ProclinConstituents: PBS. pH 7.4 |
