Application
C. Immunofluorescent staining of A431 cells using anti-Phospho-EGFR (pY1068) RabMAb (cat. #).Application
A. Western blot analysis on A431 cell lysate using anti-Phospho-EGFR (pY1068) RabMAb (cat. #); dilution 1:10000. Cell lysates were either (1) untreated or (2) treated with EGF.| Product Name | EGFR (phospho Tyr1068) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [Y38] to EGFR (phospho Tyr1068)Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation . Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor . The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding . Once activated, EGFR phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. Additionally, phosphotyrosine Y-1173 is a major binding site for SHC on the EGFR . |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGF receptor was used as immunogen. |
| Modification | p-Tyr1068 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | Y38 |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIPWB |
| Recommended Starting Dilutions:
For WB: Use at a dilution of 1:5000 - 10000
For ICC: Use at a dilution of 1:100-1:250
For FACS: Use at a dilution of 1:40
For IP: Use at a dilution of 1:150
Optimal working dilution for a specific application should be determined by the investigator.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERBB1 HER1 EGFR ERBB mENA PIG61 EGFR (phospho Y1068) EGFR (phospho Tyr1068) EGFR (pY1068) EGFR (pTyr1068) EGFR phospho Y1068 EGFR phospho Tyr1068 Phospho EGFR (Y1068) Phospho EGFR (Tyr1068) Phospho EGFR (pY1068) Phospho EGFR (pTyr1068) |
|---|---|
| Target | EGFR Phospho (pY1068) |
| Molecular Weight(MW) | 170 (note) |
| Tissue Specificity | recognizes only EGFR phosphorylated on Tyrosine 1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor form P00533-1/p170). |
Application
C. Immunofluorescent staining of A431 cells using anti-Phospho-EGFR (pY1068) RabMAb (cat. #).
Application
A. Western blot analysis on A431 cell lysate using anti-Phospho-EGFR (pY1068) RabMAb (cat. #); dilution 1:10000. Cell lysates were either (1) untreated or (2) treated with EGF.| Application Notes | Recommended Starting Dilutions:
For WB: Use at a dilution of 1:5000 - 10000
For ICC: Use at a dilution of 1:100-1:250
For FACS: Use at a dilution of 1:40
For IP: Use at a dilution of 1:150
Optimal working dilution for a specific application should be determined by the investigator.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
