Application
Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR(Phospho-Tyr1197) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Phospho-Tyr1197) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR (Phospho-Tyr1197) Antibody .| Product Name | EGFR (phospho Tyr1197) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EGFR (Phospho Tyr1197)Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 1197 (A-E-Y(p)-L-R) derived from Human EGFR. |
| Modification | p-Tyr1197 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ERBB ERBB1 HER1 PIG61 mENA EGFR receptor tyrosine-protein kinase erbB-1 cell growth inhibiting protein 40 epidermal growth factor receptor proto-oncogene c-ErbB-1 avian erythroblastic leukemia viral (v-erb-b) oncogene homolog cell proliferation- |
|---|---|
| Target | EGFR (Phospho Tyr1197) |
| Molecular Weight(MW) | 175 (note) |
Application
Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR(Phospho-Tyr1197) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Phospho-Tyr1197) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR (Phospho-Tyr1197) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
