Application
Western blot using EGFR [pY845] polyclonal antibody, rabbit.| Product Name | EGFR (phospho Tyr845) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EGFR (phospho Y845)Epidermal Growth Factor Receptor, also known as ErbB 1 (EGFR, a 185 kDa glycoprotein) is a transmembrane tyrosine kinase that regulates a variety of biological responses ranging from mitogenesis to stress signaling. The EGFR consists of a large extracellular domain, a single transmembrane domain and a cytoplasmic domain that exhibits kinase activity. Upon binding of EGF to the extracellular domain, the receptor undergoes dimerization and becomes phosphorylated on several tyrosine residues within the cytoplasmic domain. These events result in EGFR activation and increased tyrosine kinase activity toward a variety of intracellular substrates. Tyrosine 845 a major autophosphorylation site in EGFR is mediated by Src, and regulates receptor function and tumor progression. |
| Immunogen | Synthetic phosphopeptide derived from the region of EGFR that contains tyrosine 845. |
| Modification | p-Tyr845 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC: Use at an assay dependent dilution. WB: 1/1000. Detects a band of approximately 185 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERBB1 131550 1956 EGFR ERBB mENA P00533 EGFR (phospho Y845) EGFR (phospho Tyr845) EGFR (pY845) EGFR (pTyr845) EGFR phospho Y845 EGFR phospho Tyr845 Phospho EGFR (Y845) Phospho EGFR (Tyr845) Phospho EGFR (pY845) Phospho EGFR (pTyr845) |
|---|---|
| Target | EGFR |
| Cellular Localization | Cell Membrane and Secreted |
Application
Western blot using EGFR [pY845] polyclonal antibody, rabbit.| Positive Control | Human Epidermoid Carcinoma (A431) cells and cortic smooth muscle cells |
|---|---|
| Application Notes | ICC: Use at an assay dependent dilution. WB: 1/1000. Detects a band of approximately 185 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol 1mg/ml BSA. pH 7.3 |
