Application
Immunofluorescence staining of methanol-fixed MCF cells using EGFR Antibody .Application
Western blot analysis of extracts from HUVEC and MDA cells using EGFR Antibody .Application
Western blot analysis of extracts from HUVEC and MDA cells using EGFR(Ab-1070) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | EGFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EGFRReceptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia |
| Immunogen | Peptide sequence around aa.1068~1072 (R-Y-S-S-D) derived from Human EGFR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ERBB ERBB1 HER1 PIG61 mENA EGFR receptor tyrosine-protein kinase erbB-1 cell growth inhibiting protein 40 epidermal growth factor receptor proto-oncogene c-ErbB-1 avian erythroblastic leukemia viral (v-erb-b) oncogene homolog cell proliferation- |
|---|---|
| Target | EGFR |
| Molecular Weight(MW) | 175 (note) |
Application
Immunofluorescence staining of methanol-fixed MCF cells using EGFR Antibody .
Application
Western blot analysis of extracts from HUVEC and MDA cells using EGFR Antibody .
Application
Western blot analysis of extracts from HUVEC and MDA cells using EGFR(Ab-1070) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
