Application
A. Western blot analysis of A431 cell lysate using anti EGFR RabMab (cat# 1114 -1).Application
B. Immunohistochemical analysis of human cervical carcinoma using anti EGFR RabMab (cat# 1114 -1).Application
C. Immunofluorescent staining of A431 cells using anti EGFR RabMab (cat# 1114 -1).| Product Name | EGFR antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [E114] to EGFREpidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation . Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor . Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal of EGFR . Autophosphorylation of Tyr 992 creates a binding site for the phospholipase C-gamma (PLC-gamma) SH2 domain, inducing downstream signaling . |
| Immunogen | A synthetic peptide corresponding to residues near C-terminus of human EGF receptor was used as immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | E114 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| For WB: Use at a dilution of 1:1,000 - 10,000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | ERBB1 HER1 EGFR ERBB mENA PIG61 |
|---|---|
| Target | EGFR |
| Molecular Weight(MW) | 170-180 (note) |
Application
A. Western blot analysis of A431 cell lysate using anti EGFR RabMab (cat# 1114 -1).
Application
B. Immunohistochemical analysis of human cervical carcinoma using anti EGFR RabMab (cat# 1114 -1).
Application
C. Immunofluorescent staining of A431 cells using anti EGFR RabMab (cat# 1114 -1).| Application Notes | For WB: Use at a dilution of 1:1,000 - 10,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
