Application
Formalin fixed paraffin embedded human placenta stained with EGFR antibodyApplication
Flow cytometric analysis of rabbit anti-EGFR (SP9) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)Application
Human placenta stained with anti-Epidermal Growth Factor Receptor (EGFR) antibody| Product Name | EGFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal [SP9] to EGFREpidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. EGFR over expression in tumors indicates poor prognosis. EGFR over expression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. |
| Immunogen | Synthetic peptide (Human) (C terminal). |
| Clonality | Monoclonal |
|---|---|
| Clone Number | SP9 |
| Host Species | Rabbit |
| Tested Applications | FACSIHCIHC-PWB |
| IHC-P: 1/100. Antigen retrieval is not essential but may optimise staining. WB: 1/200. Predicted molecular weight: 134 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERBB1 131550 1956 EGFR ERBB mENA P00533 |
|---|---|
| Target | EGFR |
| Cellular Localization | Cell Membrane and Secreted |
Application
Formalin fixed paraffin embedded human placenta stained with EGFR antibody
Application
Flow cytometric analysis of rabbit anti-EGFR (SP9) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)
Application
Human placenta stained with anti-Epidermal Growth Factor Receptor (EGFR) antibody| Positive Control | Placenta and lung |
|---|---|
| Application Notes | IHC-P: 1/100. Antigen retrieval is not essential but may optimise staining. WB: 1/200. Predicted molecular weight: 134 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.1% Sodium Azide |
