Application
Formalin fixed paraffin embedded human placenta stained with EGFR antibody (cat. # ).Application
Flow cytometric analysis of rabbit anti-EGFR (SP9) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)Application
Human placenta stained with anti-Epidermal Growth Factor Receptor (EGFR) antibody| Product Name | EGFR antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Rabbit Antibody to Human EGFRThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | A synthetic peptide from C-terminus of human EGFR protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | SP9 |
| Host Species | Rabbit |
| Tested Applications | FACSIHCIHC-P |
| We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. No pretreatment is required for formalin fixed paraffin tissue sections.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EGFR P00533 ERBB1 131550 1956 ERBB mENA HER1 PIG61 |
|---|---|
| Target | EGFR |
| Tissue Specificity | This antibody recognizes EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, 02, 03, -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.This antibody crossreacts with Human. |
| Cellular Localization | Cytoplasmic |
Application
Formalin fixed paraffin embedded human placenta stained with EGFR antibody (cat. # ).
Application
Flow cytometric analysis of rabbit anti-EGFR (SP9) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)
Application
Human placenta stained with anti-Epidermal Growth Factor Receptor (EGFR) antibody| Positive Control | Placenta, Lung |
|---|---|
| Application Notes | We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. No pretreatment is required for formalin fixed paraffin tissue sections.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | The buffer contains BSA and 0.05% sodium azide. |
