Application
Immunohistochemical analysis of mouse brain tissue (paraformaldehyde-fixed frozen sections), using EGR2 antibody at 1:500 dilution.Application
Western Blot analysis of EGR2 expression in transfected 293T cell line by EGR2 polyclonal antibody. A: Non-transfected lysate. B: EGR2 transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | EGR2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EGR2 (early growth response 2)The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EGR2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus MonkeyRat (88%), Pig (95%), Rhesus Monkey (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AT591 antibody CMT1D antibody CMT4E antibody DKFZp686J1957 antibody FLJ14547 antibody KROX20 antibody EGR2 antibody EGR-2 antibody early growth response protein 2 antibody "Krox-20 homolog Drosophila antibody" zinc finger protein Krox-20 antibod |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of mouse brain tissue (paraformaldehyde-fixed frozen sections), using EGR2 antibody at 1:500 dilution.
Application
Western Blot analysis of EGR2 expression in transfected 293T cell line by EGR2 polyclonal antibody. A: Non-transfected lysate. B: EGR2 transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | EGR2 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
