Application
Western blot analysis of EHD3 Antibody (Center) in CEM cell line lysates (35ug/lane). EHD3 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of 293 cells using EHD3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with EHD3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EHD3 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EHD3 (Internal)Plays a role in endocytic transport. |
| Immunogen | KLH conjugated synthetic peptide between 195~225 amino acids from the Center region of human EHD3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | EH domaincontaining protein 3 EH domain-containing protein 3 EHD3 synonyms: EHD2 EH domaincontaining protein3 name: EHD3 PAST3 EH domain containing protein 3 |
|---|---|
| Target | EHD3 |
| Molecular Weight(MW) | 61.896 kDa (note) |
Application
Western blot analysis of EHD3 Antibody (Center) in CEM cell line lysates (35ug/lane). EHD3 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of 293 cells using EHD3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with EHD3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
