Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using eIF2B epsilon antibody at 1:200 dilution.| Product Name | EIF2B epsilon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF2B epsilon (eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa)This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF2B epsilon. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rabbit, Rhesus MonkeyRat (90%), Rabbit (92%), Rhesus Monkey (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CACH antibody, CLE antibody, EIF-2B antibody, EIF2Bepsilon antibody, LVWM antibody, EIF2B5 antibody, translation initiation factor eIF-2B subunit epsilon antibody, eIF-2B GDP-GTP exchange factor subunit epsilon antibody, "eukaryotic translation initiation |
|---|---|
| Molecular Weight(MW) | 80 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using eIF2B epsilon antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
