Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 12% SDS PAGE diluted at 1:10000Application
EIF2B1 antibody detects EIF2B1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2B1 protein stained by EIF2B1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EIF2B1 antibody at 1:500 dilution.| Product Name | EIF2B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF2B1 (eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa)This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq] |
| Immunogen | Full length human EIF2B1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (94%), Rat (92%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EIF2B antibody EIF2BA antibody MGC117409 antibody MGC125868 antibody MGC125869 antibody EIF2B1 antibody eIF-2B GDP-GTP exchange factor subunit alpha antibody translation initiation factor eIF-2B subunit alpha antibody "eukaryotic translation initia |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 12% SDS PAGE diluted at 1:10000
Application
EIF2B1 antibody detects EIF2B1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2B1 protein stained by EIF2B1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EIF2B1 antibody at 1:500 dilution.| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
