Application
Sample (30 ug of whole cell lysate) A: A431 B: HepG2 10% SDS PAGE diluted at 1:1000Application
EIF3F antibody [N1C3] detects EIF3F protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EIF3F antibody [N1C3] dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EIF3F antibody at 1:100 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using EIF3F antibody at 1:200 dilution.| Product Name | EIF3F antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF3F (eukaryotic translation initiation factor 3, subunit F)Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF3F. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, ChimpanzeeRat (99%), Chimpanzee (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF3S5 antibody, eIF3-p47 antibody, EIF3F antibody, "eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa antibody", eIF3-epsilon antibody, "eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD) antibody", eukaryotic transla |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: A431 B: HepG2 10% SDS PAGE diluted at 1:1000
Application
EIF3F antibody [N1C3] detects EIF3F protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EIF3F antibody [N1C3] dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EIF3F antibody at 1:100 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using EIF3F antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
