Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 C:Hep G2 10% SDS PAGE diluted at 1:1000| Product Name | eIF3H / EIF3S3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to EIF3S3 (eukaryotic translation initiation factor 3, subunit H)Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF3 gamma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (96%), Rat (97%), Chicken (90%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF3S3 antibody, MGC102958 antibody, eIF3-gamma antibody, eIF3-p40 antibody, EIF3H antibody, eukaryotic translation initiation factor 3 subunit 3 antibody, eIF-3-gamma antibody, eukaryotic translation initiation factor 3 subunit H antibody, "eukaryotic tra |
|---|---|
| Target | EIF3S3 |
| Molecular Weight(MW) | 40 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 C:Hep G2 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
