Application
Immunofluorescence analysis of methanol-fixed HeLa, using EIF4A1 antibody at 1:500 dilution.Application
Immunoprecipitation of EIF4A1 protein from HepG2 whole cell extracts using 5 μg of EIF4A1 antibody . Western blot analysis was performed using EIF4A1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:3000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF4A1 antibody at 1:500 dilution.| Product Name | EIF4A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF4A1 (eukaryotic initiation factor 4A-I) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF4A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Pig, Chimpanzee, BovineMouse (100%), Rat (100%), Xenopus laevis (96%), Dog (100%), Pig (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DDX2A antibody EIF-4A antibody EIF4A antibody eIF-4A-I antibody eIF4A-I antibody EIF4A1 antibody eukaryotic initiation factor 4AI antibody ATP-dependent RNA helicase eIF4A-1 antibody "eukaryotic translation initiation factor 4A isoform 1 antibody" |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (2) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using EIF4A1 antibody at 1:500 dilution.
Application
Immunoprecipitation of EIF4A1 protein from HepG2 whole cell extracts using 5 μg of EIF4A1 antibody . Western blot analysis was performed using EIF4A1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:3000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF4A1 antibody at 1:500 dilution.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
