Application
Sample(30 μg of whole cell lysate) A:293T B:A431 12% SDS PAGE diluted at 1:200| Product Name | EIF4A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to eIF4A2 (eukaryotic translation initiation factor 4A, isoform 2)ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human EIF4A2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (100%), Rat (100%), Zebrafish (95%), Pig (100%), Chicken (95%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BM-010 antibody DDX2B antibody EIF4A antibody EIF4F antibody eIF-4A-II antibody eIF4A-II antibody EIF4A2 antibody "eukaryotic translation initiation factor 4A isoform 2 antibody" ATP-dependent RNA helicase eIF4A-2 antibody eukaryotic initiation f |
|---|---|
| Target | EIF4A2 |
| Molecular Weight(MW) | 46 kDa (note) |
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 12% SDS PAGE diluted at 1:200| Positive Control | 293T , A431 , HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
