Application
Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using eIF4E(Phospho-Ser209) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209) Antibody .Application
Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209) Antibody .| Product Name | eIF4E (phospho Ser209) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eIF4E (Phospho Ser209)Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. |
| Immunogen | Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E. |
| Modification | p-Ser209 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CBP EIF4E1 EIF4EL1 EIF4F MGC111573 EIF4E eukaryotic translation initiation factor 4E-like 1 eukaryotic translation initiation factor 4E eIF-4F 25 kDa subunit eIF-4E mRNA cap-binding protein |
|---|---|
| Target | eIF4E (Phospho Ser209) |
| Molecular Weight(MW) | 25 (note) |
Application
Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using eIF4E(Phospho-Ser209) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209) Antibody .
Application
Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
