Application
Extracts of HeLa cells were resolved by SDS-PAGE on a 4-20% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, either left untreated (1-4) or treated with lambda phosphatase (5), and then incubated with the EIF-4E [pS209] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1,5), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that only the phosphopeptide corresponding to EIF-4E [pS209] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, further verifying that the antibody is phospho-specific.| Product Name | eIF4E (phospho Ser209) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to eIF4E ( phospho S209 )eIF-4E, a protein with Mw 25 kDa, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF-4E also influences the overall rate of translation. eIF-4E binds to the 7-methyl-GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF-4E on serine 209 regulates the affinity of this protein for the 7-methyl-GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF-4E with eIF-4G, which form a complex known as eIF-4F. eIF-4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF-4E including PKC and/or the MAP kinase-activated Mnk. |
| Immunogen | Synthetic peptide (Human) derived from the region of eIF-4E that contains serine 209, based on the Homo sapiens sequence. This region is identical among many species including rat, mouse and rabbit. |
| Modification | p-Ser209 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.2 - 1.0 μg/ml. Predicted molecular weight: 25 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CBP EIF4E EIF4EL1 P06730 MGC111573 EIF4F 1977 133440 EIF4E (phospho S209) EIF4E (phospho Ser209) EIF4E (pS209) EIF4E (pSer209) EIF4E phospho S209 EIF4E phospho Ser209 Phospho EIF4E (S209) Phospho EIF4E (Ser209) Phospho EIF4E (pS209) Phosp |
|---|---|
| Target | EIF4E |
| Tissue Specificity | This phosphorylation site specific antibody is selective for eIF-4E containing a phosphate on serine 209. |
Application
Extracts of HeLa cells were resolved by SDS-PAGE on a 4-20% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, either left untreated (1-4) or treated with lambda phosphatase (5), and then incubated with the EIF-4E [pS209] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1,5), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that only the phosphopeptide corresponding to EIF-4E [pS209] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, further verifying that the antibody is phospho-specific.| Application Notes | WB: Use at a concentration of 0.2 - 1.0 μg/ml. Predicted molecular weight: 25 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage, please keep at -70°C or below. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 1% BSA, PBS. pH 7.4 |
