Application
eIF4E antibody detects EIF4E protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. eIF4E antibody diluted at 1:500.Application
eIF4E antibody detects eIF4E protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF4E protein stained by eIF4E antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody diluted at 1:1000.Application
293T whole cell and cytoplasm extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody diluted at 1:1000.| Product Name | eIF4E antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eIF4E (eukaryotic translation initiation factor 4E)All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Rabbit, Rhesus Monkey, Chimpanzee, BovineRat (99%), Xenopus laevis (91%), Rabbit (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AUTS19 EIF4E1 EIF4EL1 EIF4E EIF4F eukaryotic translation initiation factor 4E CBP |
|---|---|
| Target | eIF4E |
| Molecular Weight(MW) | 25 kDa (note) (3) |
Application
eIF4E antibody detects EIF4E protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. eIF4E antibody diluted at 1:500.
Application
eIF4E antibody detects eIF4E protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF4E protein stained by eIF4E antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody diluted at 1:1000.
Application
293T whole cell and cytoplasm extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody diluted at 1:1000.| Positive Control | 293T , 293T cytoplasm fraction extract , mouse colon |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
