Application
Western blot analysis of EIF4E Antibody in HL-60 cell line lysates (35ug/lane). EIF4E (arrow) was detected using the purified Pab.Application
EIF4E Antibody flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with APOL5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | eIF4E antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EIF4EAll eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 5-kD polypeptide, termed EIF4A (see MIM 6112), that possesses ATPase and RNA helicase activities, and a 22-kD polypeptide, EIF4G (MIM 6495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM] |
| Immunogen | a recombinant protein from human VGFR2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | eIF4E mRNA cap-binding protein mRNA capbinding protein EIF4F EIF4EL1 EIF4E Eukaryotic translation initiation factor 4E eIF-4E eIF4F 25 kDa subunit Eukaryotic translation initiation factor 4E eIF4E eIF-4F 25 kDa subunit Eukaryotic translation in |
|---|---|
| Target | EIF4E |
| Molecular Weight(MW) | 25.097 kDa (note) |
Application
Western blot analysis of EIF4E Antibody in HL-60 cell line lysates (35ug/lane). EIF4E (arrow) was detected using the purified Pab.
Application
EIF4E Antibody flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with APOL5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
