Application
EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. EIF4G3 antibody [N1], N-term dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: JC B: BCL-1 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 μg of EIF4G3 antibody [N1], N-term . Western blot analysis was performed using EIF4G3 antibody [N1], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. EIF4G3 antibody [N1], N-term dilution: 1:500.Application
EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. EIF4G3 antibody [N1], N-term dilution: 1:500.| Product Name | EIF4G3 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF4G3 (eukaryotic translation initiation factor 4 gamma 3) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF4G3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | eIF-4G 3 antibody eIF4G 3 antibody eIF4GII antibody EIF4G3 antibody eIF-4-gamma 3 antibody eukaryotic translation initiation factor 4 gamma 3 antibody eIF-4-gamma II antibody "eukaryotic translation initiation factor 4 gamma 3 antibody" |
|---|---|
| Molecular Weight(MW) | 177 kDa (note) (5) |
Application
EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. EIF4G3 antibody [N1], N-term dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: JC B: BCL-1 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 μg of EIF4G3 antibody [N1], N-term . Western blot analysis was performed using EIF4G3 antibody [N1], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. EIF4G3 antibody [N1], N-term dilution: 1:500.
Application
EIF4G3 antibody [N1], N-term detects EIF4G3 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. EIF4G3 antibody [N1], N-term dilution: 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 , JC , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
