Application
Western blot analysis of extracts from HT29 cells untreated or treated with UV using Elk-1 (Phospho-Ser383) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1 (Phospho-Ser383) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | ELK1 (phospho Ser383) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Elk-1 (Phospho Ser383)Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein have been found for this gene. |
| Immunogen | Peptide sequence around phosphorylation site of serine 383 (T-L-S(p)-P-I) derived from Human Elk-1. |
| Modification | p-Ser383 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ELK1 ETS-like gene 1 ETS domain-containing protein Elk-1 ELK1 member of ETS oncogene family |
|---|---|
| Target | Elk-1 (Phospho Ser383) |
| Molecular Weight(MW) | 47 (note) |
Application
Western blot analysis of extracts from HT29 cells untreated or treated with UV using Elk-1 (Phospho-Ser383) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1 (Phospho-Ser383) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
