| Product Name | ENC1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ENC1 (ectodermal-neural cortex (with BTB-like domain))DNA damage and/or hyperproliferative signals activate wildtype p53 tumor suppressor protein (TP53; MIM 191170), inducing cell cycle arrest or apoptosis. Mutations that inactivate p53 occur in 50% of all tumors. Polyak et al. (1997) [PubMed 9305847] used serial analysis of gene expression (SAGE) to evaluate cellular mRNA levels in a colorectal cancer cell line transfected with p53. Of 7,202 transcripts identified, only 14 were expressed at levels more than 10-fold higher in p53-expressing cells than in control cells. Polyak et al. (1997) [PubMed 9305847] termed these genes 'p53-induced genes,' or PIGs, several of which were predicted to encode redox-controlling proteins. They noted that reactive oxygen species (ROS) are potent inducers of apoptosis. Flow cytometric analysis showed that p53 expression induces ROS production, which increases as apoptosis progresses under some conditions. The authors stated that the PIG10 gene, also called ENC1, encodes an actin-binding protein.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENC1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Predicted Reactivity | Human, Mouse, Rat, Xenopus laevis, Rhesus Monkey, BovineHuman (100%), Mouse (98%), Rat (98%), Xenopus laevis (94%), Rhesus Monkey (99%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCL28 antibody, ENC-1 antibody, FLJ39259 antibody, KLHL35 antibody, KLHL37 antibody, NRPB antibody, PIG10 antibody, TP53I10 antibody, ENC1 antibody, "nuclear restricted protein, BTB domain-like (brain) antibody", nuclear matrix protein NRP/B antibody, kelc |
|---|---|
| Target | ENC1 |
| Molecular Weight(MW) | 66 kDa (note) |
| Cellular Localization | Nucleus matrix , Cytoplasm , cytoskeleton |
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
