Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Endothelin A Receptor antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: GL261 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Endothelin A Receptor antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Endothelin A Receptor (endothelin receptor type A)This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Endothelin A Receptor. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB:1:500~1:2000 IHC-p:1:50~1:500 *Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseChicken |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ETA antibody, ETAR antibody, ETRA antibody, EDNRA antibody, "endothelin receptor, ET1-specific type antibody", endothelin receptor subtype A antibody, endothelin A receptor antibody, endothelin ET-A receptor antibody, G protein-coupled receptor antibody, e |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) (3) |
| Cellular Localization | Cell membrane |
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Endothelin A Receptor antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: GL261 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | WB:1:500~1:2000 IHC-p:1:50~1:500 *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
